Lysophosphatidylcholine containing docosahexaenoic acid at the sn-1 position is anti-inflammatory

Lipids. 2010 Mar;45(3):225-36. doi: 10.1007/s11745-010-3392-5. Epub 2010 Feb 18.

Abstract

Lysophosphatidylcholine is known to be a lipid mediator in various cellular responses. In this study, we examined the anti-inflammatory actions of lysophosphatidylcholine containing docosahexaenoic acid esterified at the sn-1 position. First, in RAW 264.7 cells, DHA-lysoPtdCho suppressed the LPS-induced formation of NO concentration-dependently. However, ARA-lysoPtdCho showed a partial suppression, and LNA-lysoPtdCho had no significant effect. Additionally, DHA-lysoPtdCho also reduced the level of TNF-alpha or IL-6, but not PGE(2). In animal experiments, the i.v. administration of ARA-lysoPtdCho (150 or 500 mug/kg) prevented zymosan A-induced plasma leakage remarkably with a maximal efficacy (Emax) of 50%, in contrast to no effect with LNA-lysoPtdCho. Remarkably, DHA-lysoPtdCho suppressed zymosan A-induced plasma leakage with an ED(50) value of 46 mug/kg and an Emax value of around 95%. Additionally, mechanistic studies indicated that the anti-inflammatory action of DHA-lysoPtdCho was partially related to the reduced formation of LTC(4,) TNF-alpha, and IL-6. When the interval time between lysoPtdCho administration and zymosan A challenge was extended up to 2 h, such a suppressive action of DHA-lysoPtdCho was augmented, suggesting that a DHA-lysoPtdCho metabolite is important for anti-inflammatory action. In support of this, 17-HPDHA-lysoPtdCho showed a greater anti-inflammatory action than DHA-lysoPtdCho. Furthermore, a similar anti-inflammatory action was also observed with i.p. administration of DHA-lysoPtdCho or a 17(S)-hydroperoxy derivative. Additionally, oral administration of DHA-lysoPtdCho also expressed a significant anti-inflammatory action. Taken together, it is proposed that DHA-lysoPtdCho and its metabolites may be anti-inflammatory lipids in vivo systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents* / chemistry
  • Anti-Inflammatory Agents* / pharmacology
  • Cell Line
  • Dinoprostone / immunology
  • Docosahexaenoic Acids* / chemistry
  • Docosahexaenoic Acids* / pharmacology
  • Interleukin-6 / immunology
  • Lipopolysaccharides / immunology
  • Lipopolysaccharides / pharmacology
  • Lysophosphatidylcholines* / chemistry
  • Lysophosphatidylcholines* / pharmacology
  • Macrophages / cytology
  • Macrophages / drug effects*
  • Macrophages / immunology
  • Male
  • Mice
  • Nitric Oxide / metabolism
  • Tumor Necrosis Factor-alpha / immunology
  • Zymosan / immunology
  • Zymosan / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Interleukin-6
  • Lipopolysaccharides
  • Lysophosphatidylcholines
  • Tumor Necrosis Factor-alpha
  • Docosahexaenoic Acids
  • Nitric Oxide
  • Zymosan
  • Dinoprostone