Overexpression of MIP2, a novel WD-repeat protein, promotes proliferation of H9c2 cells

Biochem Biophys Res Commun. 2010 Mar 19;393(4):860-3. doi: 10.1016/j.bbrc.2010.02.099. Epub 2010 Feb 18.


WD40 repeat proteins have a wide range of diverse biological functions including signal transduction, cell cycle regulation, RNA splicing, and transcription. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a novel member of the WD40 repeat proteins superfamily that contains five WD40 repeats. Little is known about its biological role, and the purpose of this study was to determine the role of MIP2 in regulating cellular proliferation. Transfection and constitutive expression of MIP2 in the rat cardiomyoblast cell line H9c2 results in enhanced growth of those cells as measured by cell number and is proportional to the amount of MIP2 expressed. Overexpression of MIP2 results in a shorter cell cycle, as measured by flow cytometry. Collectively, these data suggest that MIP2 may participate in the progression of cell proliferation in H9c2 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Cell Cycle
  • Cell Line
  • Cell Proliferation*
  • Humans
  • Molecular Sequence Data
  • Proteins / genetics
  • Proteins / physiology*
  • Rats


  • Adaptor Proteins, Signal Transducing
  • Proteins
  • WDR26 protein, human