Abstract
Der, an essential Escherichia coli tandem GTPase, has been implicated in 50S subunit biogenesis. The rrmJ gene encodes a methyltransferase that modifies the U2552 residue of 23S rRNA, and its deletion causes a severe growth defect. Peculiarly, overexpression of Der suppresses growth impairment. In this study, using an rrmJ-deletion strain, we demonstrated that two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. We also identified a region of Der that is critical for its specific interaction with 50S subunit.
MeSH terms
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / physiology
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / metabolism*
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Methyltransferases / genetics
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Methyltransferases / physiology
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Mutagenesis
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Phenotype
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Protein Binding / genetics
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Protein Binding / physiology
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Protein Structure, Tertiary / genetics
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Protein Structure, Tertiary / physiology
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Ribosome Subunits, Large, Bacterial / genetics
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Ribosome Subunits, Large, Bacterial / metabolism*
Substances
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Cell Cycle Proteins
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Escherichia coli Proteins
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Methyltransferases
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rlmE protein, E coli
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Der protein, E coli
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GTP-Binding Proteins