Microwave-assisted extraction followed by CE for determination of catechin and epicatechin in green tea

J Sep Sci. 2010 Apr;33(8):1079-84. doi: 10.1002/jssc.200900647.


In this work, for the first time, microwave-assisted extraction (MAE) followed by CE was developed for the fast analysis of catechin and epicatechin in green tea. In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. The MAE conditions and the method's validation were studied. It is found that the extraction time of 1 min with 400 W microwave irradiation is enough to completely extract catechin and epicatechin in green tea sample, whereas the conventional ultrasonic extraction (USE) technique needs long extraction time of 60 min. The method validations were also studied in this work. The calibration curve shows good linearity in 0.01-3 mg/mL for catechin (R(2)=0.993), and 0.005-3 mg/mL for epicatechin (R(2)=0.996), respectively. The RSD values for catechin and epicatechin are 0.65 and 2.58%, respectively. This shows that the proposed method has good reproducibility. The proposed method has good recoveries, which are 118% for catechin and 120% for epicatechin. The proposed method was successfully applied to determination of the catechin and epicatechin in different green tea samples. The experiment results have demonstrated that the MAE following CE is a simple, fast and reliable method for the determination of catechin and epicatechin in green tea.

MeSH terms

  • Catechin / analysis*
  • Electrophoresis, Capillary / instrumentation
  • Microwaves*
  • Molecular Conformation
  • Reproducibility of Results
  • Stereoisomerism
  • Tea / chemistry*


  • Tea
  • Catechin