Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

Mol Biol Rep. 2010 Oct;37(7):3567-74. doi: 10.1007/s11033-010-0005-6. Epub 2010 Feb 24.


MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genome / genetics
  • MicroRNAs / chemistry
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Muscle, Skeletal / metabolism*
  • Nucleic Acid Conformation
  • RNA Precursors / chemistry
  • RNA Precursors / genetics
  • Sequence Analysis, RNA
  • Sus scrofa / genetics*


  • MicroRNAs
  • RNA Precursors