85-kDa protein of Trypanosoma cruzi purified by affinity chromatography used in the multiple antigen binding assay (MABA) for the diagnosis of T. cruzi infection in a Venezuelan rural community

Parasitol Res. 2010 Apr;106(5):1127-34. doi: 10.1007/s00436-010-1773-6. Epub 2010 Feb 24.


No ideal test exists for Chagas' disease, and better diagnostic strategies are needed. We determined the diagnostic utility of an 85-kDa Trypanosoma cruzi protein in a multiple antigen binding assay (MABA). A standardized MABA test based on concentrated trypomastigote excretory-secretory antigen (TESA) and an 85-kDa purified protein showed 100% sensitivity and specificity. In field conditions, 6/66 individuals tested in a region not thought to be endemic (Rio Brito) were identified as seropositive for T. cruzi infection with our MABA test. In parallel, an enzyme-linked immunosorbent assay based on fixed epimastigotes detected 7/66 positives, which were independently confirmed. These data suggest that the 85-kDa and TESA proteins could be used in the MABA format as a complementary tool for the diagnosis of latent Chagas' disease. High anti-T. cruzi antibody detection rates, poor knowledge of Chagas' disease and its vector, and the demonstration of infected vectors in the study community all suggest a significant risk of reemergence of T. cruzi infection in this region of Venezuela.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antigens, Protozoan* / isolation & purification
  • Chagas Disease / diagnosis*
  • Chagas Disease / parasitology
  • Child
  • Chromatography, Affinity
  • Clinical Laboratory Techniques / methods*
  • Female
  • Humans
  • Immunoassay / methods
  • Male
  • Membrane Glycoproteins* / isolation & purification
  • Middle Aged
  • Protozoan Proteins* / isolation & purification
  • Rural Population
  • Sensitivity and Specificity
  • Trypanosoma cruzi / immunology
  • Trypanosoma cruzi / isolation & purification*
  • Venezuela
  • Young Adult


  • Antigens, Protozoan
  • Membrane Glycoproteins
  • Protozoan Proteins
  • SA85 1.1 antigen, Trypanosoma cruzi