Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

FASEB J. 2010 Jul;24(7):2245-53. doi: 10.1096/fj.09-137174. Epub 2010 Feb 24.


Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6(+) cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Lineage
  • Cell Separation
  • Induced Pluripotent Stem Cells / cytology*
  • Mice
  • Muscle Cells / cytology
  • Muscle, Skeletal / cytology*
  • Regeneration
  • Stem Cell Transplantation
  • Stem Cells / cytology*
  • Stem Cells / physiology