Global transcriptomic analysis of murine embryonic stem cell-derived brachyury(+) (T) cells

Genes Cells. 2010 Mar;15(3):209-28. doi: 10.1111/j.1365-2443.2010.01390.x. Epub 2010 Feb 24.

Abstract

Brachyury(+) mesodermal cell population with purity over 79% was obtained from differentiating brachyury embryonic stem cells (ESC) generated with brachyury promoter driven enhanced green fluorescent protein and puromycin-N-acetyltransferase. A comprehensive transcriptomic analysis of brachyury(+) cells enriched with puromycin application from 6-day-old embryoid bodies (EBs), 6-day-old control EBs and undifferentiated ESCs led to identification of 1573 uniquely up-regulated and 1549 uniquely down-regulated transcripts in brachyury(+) cells. Furthermore, transcripts up-regulated in brachyury(+) cells have overrepresented the Gene Ontology annotations (cell differentiation, blood vessel morphogenesis, striated muscle development, placenta development and cell motility) and Kyoto Encyclopedia of Genes and Genomes pathway annotations (mitogen-activated protein kinase signaling and transforming growth factor beta signaling). Transcripts representing Larp2 and Ankrd34b are notably up-regulated in brachyury(+) cells. Knockdown of Larp2 resulted in a significantly down-regulation BMP-2 expression, and knockdown of Ankrd34b resulted in alteration of NF-H, PPARγ and PECAM1 expression. The elucidation of transcriptomic signatures of ESCs-derived brachyury(+) cells will contribute toward defining the genetic and cellular identities of presumptive mesodermal cells. Furthermore, there is a possible involvement of Larp2 in the regulation of the late mesodermal marker BMP-2. Ankrd34b might be a positive regulator of neurogenesis and a negative regulator of adipogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / metabolism
  • Animals
  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Bone Morphogenetic Protein 2 / metabolism
  • Cells, Cultured
  • Embryoid Bodies / cytology
  • Embryoid Bodies / metabolism
  • Embryonic Stem Cells / metabolism*
  • Fetal Proteins / metabolism*
  • Mice
  • Neurofilament Proteins / metabolism
  • PPAR gamma / metabolism
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
  • Promoter Regions, Genetic
  • RNA, Small Interfering / genetics
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism
  • T-Box Domain Proteins / metabolism*
  • T-Lymphocytes / metabolism*
  • Transcriptome*

Substances

  • Autoantigens
  • Bmp2 protein, mouse
  • Bone Morphogenetic Protein 2
  • Fetal Proteins
  • Neurofilament Proteins
  • PPAR gamma
  • Platelet Endothelial Cell Adhesion Molecule-1
  • RNA, Small Interfering
  • Ribonucleoproteins
  • SS-B antigen
  • T-Box Domain Proteins
  • neurofilament protein H
  • Acetyltransferases
  • puromycin N-acetyltransferase
  • Brachyury protein