Porcine peroxisome proliferator-activated receptor delta mediates the lipolytic effects of dietary fish oil to reduce body fat deposition

J Anim Sci. 2010 Jun;88(6):2009-18. doi: 10.2527/jas.2009-2597. Epub 2010 Feb 26.


Peroxisome proliferator-activated receptor delta promotes fatty acid catabolism and energy expenditure in skeletal muscle and adipose tissues. A ligand for PPARdelta is required to activate PPARdelta function. Polyunsaturated fatty acids are potential ligands for PPARdelta activation. The current experiment was designed to determine the potential for PUFA, particularly from dietary fish oil, to activate porcine PPARdelta in vivo. Transgenic mice were generated to overexpress porcine PPARdelta in the adipose tissue. Mice were fed a high-saturated fat (13% beef tallow), or high-unsaturated fat (13% fish oil) diet, or a diet containing 4 mg/kg of a PPARdelta ligand (L165041) for 4 mo. Compared with beef tallow feeding, fish oil feeding reduced fat mass and decreased (P < 0.05) plasma triacylglycerol and FFA concentrations in the transgenic mice. Adipose tissue expression of genes involved in adipogenesis (i.e., lipoprotein lipase and adipocyte fatty acid-binding protein) was decreased in transgenic mice fed fish oil or the PPARdelta ligand. In the same mice, expression of the lipolytic gene, hormone-sensitive lipase was increased (P < 0.05). Fish oil feeding also stimulated expression of genes participating in fatty acid oxidation in the liver of transgenic mice compared with wild-type mice. Overall, these results indicate that PUFA may serve as natural and effective regulators of lipid catabolism in vivo and many of these effects may be generated from activation of PPARdelta.

MeSH terms

  • Adiponectin / genetics
  • Adiponectin / metabolism
  • Adipose Tissue / metabolism*
  • Animals
  • Body Weight / physiology
  • Dietary Fats, Unsaturated / metabolism*
  • Eating / physiology
  • Fatty Acids, Nonesterified / blood
  • Fish Oils / metabolism*
  • Ion Channels / genetics
  • Ion Channels / metabolism
  • Ligands
  • Lipolysis / physiology*
  • Lipoprotein Lipase / genetics
  • Lipoprotein Lipase / metabolism
  • Liver / metabolism
  • Mice
  • Mice, Transgenic
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • Organ Size / physiology
  • PPAR delta / metabolism*
  • Phenoxyacetates / pharmacology
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sterol Esterase / genetics
  • Sterol Esterase / metabolism
  • Sterol Regulatory Element Binding Protein 1 / genetics
  • Sterol Regulatory Element Binding Protein 1 / metabolism
  • Triglycerides / blood
  • Uncoupling Protein 3


  • 4-(3-(2-propyl-3-hydroxy-4-acetyl)phenoxy)propyloxyphenoxy acetic acid
  • Adiponectin
  • Adipoq protein, mouse
  • Dietary Fats, Unsaturated
  • Fatty Acids, Nonesterified
  • Fish Oils
  • Ion Channels
  • Ligands
  • Mitochondrial Proteins
  • PPAR delta
  • Phenoxyacetates
  • RNA, Messenger
  • Srebf1 protein, mouse
  • Sterol Regulatory Element Binding Protein 1
  • Triglycerides
  • Uncoupling Protein 3
  • Sterol Esterase
  • Lipoprotein Lipase