The mutations of Th1 cell-specific T-box transcription factor may be associated with a predominant Th2 phenotype in gastric cancers

Int J Immunogenet. 2010 Apr;37(2):111-5. doi: 10.1111/j.1744-313X.2010.00899.x. Epub 2010 Feb 19.


Gastric cancer is a serious public health cancer and causes nearly 1 million deaths a year worldwide. Th1 cells play critical roles in orchestrating the adaptive immune responses against gastric cancer. T-bet, a member of the T-box family of transcription factors, is the Th1 master regulator and up-regulated during Th1 differentiation. Polymorphisms have also been shown to exist in T-bet. Some reports indicated that some tumours were associated with the drift of Th1 and Th2. In the present work, we investigated the drift of Th1/Th2 by detecting the expression levels of T-bet, IFN-gamma, IL-4, and GATA-3 in peripheral blood mononuclear cell of gastric cancer patients by real-time PCR, explored the relationship between the polymorphism of T-bet gene and drift of Th1/Th2 by gene sequence, western blot, and gene transfection. Our results indicated that a predominant Th2 phenotype was existence. T-bet gene mutations may be associated with Th2-dominated condition in gastric cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Blotting, Western
  • Cell Line
  • Cell Line, Tumor
  • Female
  • GATA3 Transcription Factor / genetics
  • GATA3 Transcription Factor / metabolism
  • Gene Expression Profiling
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Interferon-gamma / genetics
  • Interferon-gamma / metabolism
  • Interleukin-4 / genetics
  • Interleukin-4 / metabolism
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Microscopy, Fluorescence
  • Middle Aged
  • Mutation*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • T-Box Domain Proteins / genetics*
  • T-Box Domain Proteins / metabolism
  • Th1 Cells / metabolism*
  • Th2 Cells / metabolism*


  • GATA3 Transcription Factor
  • Recombinant Fusion Proteins
  • T-Box Domain Proteins
  • T-box transcription factor TBX21
  • Green Fluorescent Proteins
  • Interleukin-4
  • Interferon-gamma