Modifications in tissue kallikrein activity with indomethacin and prednisolone treatment in arthritic rats

Inflammopharmacology. 2010 Jun;18(3):113-7. doi: 10.1007/s10787-010-0034-8. Epub 2010 Feb 27.


This investigation was conducted to evaluate the tissue kallikrein activity in the synovial and paw tissues of control, non-treated adjuvant arthritic and adjuvant arthritic rats treated either with indomethacin or prednisolone. Adjuvant arthritis was induced in male Sprague-Dawley rats in the right knee by injecting 0.05 ml of a fine suspension of heat-killed Mycobacterium tubercle bacilli in liquid paraffin (5 mg/ml). Indomethacin (2.5 mg/kg given orally) and prednisolone (3.0 mg/kg given orally) treatment for 9 days caused a significant reduction (P < 0.001) in knee swelling. In non-treated adjuvant rats, synovial tissue kallikrein levels were raised (P < 0.01) than the synovial tissue kallikrein obtained from control normal rats. Prednisolone treatment resulted in reduction (P < 0.05) and indomethacin treatment produced raise (P < 0.01) in synovial tissue kallikrein levels. In non-treated arthritic rats, the paw tissue kallikrein levels were lower (P < 0.001) than the control rats; whereas, rats treated with prednisolone and indomethacine showed higher levels (P < 0.05) of paw tissue kallikrein. These results may suggest that the prednisone and indomethacin differ in their actions in inflamed synovial tissue kallikrein and have a similar effect in non-inflamed paw tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / metabolism
  • Arthritis, Experimental / pathology
  • Hindlimb / drug effects
  • Hindlimb / metabolism
  • Hindlimb / pathology
  • Indomethacin / administration & dosage
  • Indomethacin / therapeutic use*
  • Joints / drug effects
  • Joints / metabolism
  • Joints / pathology
  • Male
  • Mycobacterium tuberculosis / immunology
  • Prednisolone / administration & dosage
  • Prednisolone / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Kallikreins / metabolism*


  • Prednisolone
  • Tissue Kallikreins
  • Indomethacin