Biosynthesis of silver and gold nanoparticles using Brevibacterium casei

Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):257-62. doi: 10.1016/j.colsurfb.2010.02.007. Epub 2010 Feb 11.


The present study demonstrates an unprecedented green process for the production of spherical-shaped Au and Ag nanoparticles synthesized and stabilized using a bacterium, Brevibacterium casei. Aqueous solutions of chloroaurate ions for Au and Ag(+) ions for silver were treated with B. casei biomass for the formation of Au nanoparticles (AuNP) and Ag nanoparticles (AgNP). The nanometallic dispersions were characterized by surface plasmon absorbance measuring at 420 and 540 nm for Ag and Au nanoparticles, respectively. Transmission electron microscopy showed the formation of nanoparticles in the range of 10-50 nm (silver), and 10-50 nm (gold). XRD analysis of the silver and gold nanoparticles confirmed the formation of metallic silver and gold. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR), provides evidence for the presence of proteins as possible biomolecules responsible for the reduction and capping agent which helps in increasing the stability of the synthesized silver and gold nanoparticles. The biological activities of the synthesized particles were confirmed based on their stable anti-coagulant effects. The use of bacterium for nanoparticles synthesis offers the benefits of ecofriendliness and amenability for large-scale production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anticoagulants / chemistry
  • Biomass
  • Blood Proteins / metabolism
  • Brevibacterium / metabolism*
  • Female
  • Gold / chemistry*
  • Humans
  • Luminescence
  • Male
  • Metal Nanoparticles / chemistry*
  • Middle Aged
  • Nanotechnology / methods*
  • Photochemistry / methods
  • Silver / chemistry*
  • Spectroscopy, Fourier Transform Infrared / methods
  • Surface Properties
  • X-Ray Diffraction


  • Anticoagulants
  • Blood Proteins
  • Silver
  • Gold