Inflammation in Vivo Is Modulated by GPR83 isoform-4 but Not GPR83 isoform-1 Expression in Regulatory T Cells

Genes Immun. 2010 Jun;11(4):357-61. doi: 10.1038/gene.2010.5. Epub 2010 Mar 4.

Abstract

Most recently, we have described the G-protein coupled receptor 83 (GPR83), which is highly expressed by CD4(+)CD25(+) regulatory T cells (Tregs) to be involved in the induction of CD4(+)Foxp3(+) Tregs in the course of an ongoing immune response. Four GPR83 isoforms have been described. Here, we have shown that GPR83 isoform-4, which differs from GPR83 isoform-1 by 20 additional aminoacids in the second cytoplasmatic loop, is predominantly expressed by Tregs. Interestingly, GPR83 isoform-4 but not GPR83 isoform-1 retrovirally transduced T cells were able to interfere with inflammatory responses in vivo. Re-analysis of GPR83 transduced T cells revealed that this in vivo acquisition of suppressive activity was associated with the induction of Treg-associated molecules including Foxp3 in GPR83 isoform-4 but not GPR83 isoform-1 transduced CD4(+) T cells under inflammatory conditions. Our results suggest that the 20 additional aminoacids within GPR83 isoform-4 are involved in Treg induction during inflammatory immune responses.

MeSH terms

  • Humans
  • Phenotype
  • Protein Isoforms / genetics
  • Protein Isoforms / physiology*
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / physiology*
  • T-Lymphocytes, Regulatory / immunology*

Substances

  • GPR83 protein, human
  • Protein Isoforms
  • Receptors, G-Protein-Coupled