Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro

Acta Virol. 2010;54(1):41-8. doi: 10.4149/av_2010_01_41.

Abstract

We describe here a contribution of virus-induced actin tails and filopodia in transmission of Ectromelia virus (ECTV) infection in permissive cells detected by the immunofluorescence and confocal microscopy. Immunoblot analysis revealed profoundly decreased beta-actin levels during ECTV replicative cycle in the infected cells 24 hrs post infection (p.i.). These results provided a basis for the further analysis of ECTV motion in the infected cells as well as for impact of ECTV infection on the cytoskeletal proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Actins / ultrastructure
  • Animals
  • BALB 3T3 Cells / ultrastructure
  • BALB 3T3 Cells / virology
  • Chlorocebus aethiops
  • Cytoskeletal Proteins / metabolism
  • Cytoskeletal Proteins / ultrastructure
  • Ectromelia virus / pathogenicity*
  • Ectromelia virus / physiology
  • Ectromelia, Infectious / virology
  • Foot / virology
  • HeLa Cells / ultrastructure
  • HeLa Cells / virology
  • Humans
  • Immunoblotting
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Vero Cells / ultrastructure
  • Vero Cells / virology

Substances

  • Actins
  • Cytoskeletal Proteins