Abstract
Membrane proteins play crucial roles in various processes. However, biochemical characterization of the membrane proteins remains challenging due to the difficulty in producing membrane proteins in a functional state. Here, we describe a novel method for the production of functional membrane proteins based on a wheat germ cell-free translation system. Using this method, functional membrane proteins are successfully synthesized in the presence of liposomes and a detergent. In addition, the synthesized membrane proteins are easily purified from the cell-free translation mixture as proteoliposomes by sucrose density gradient ultracentrifugation. These advantages over conventional approaches are very helpful for the clarification of the function of membrane proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis Proteins / biosynthesis*
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / isolation & purification
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Cell-Free System
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Centrifugation, Density Gradient
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Detergents / chemistry
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Liposomes
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Membrane Transport Proteins / biosynthesis*
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Membrane Transport Proteins / chemistry
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / isolation & purification
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Protein Biosynthesis
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Protein Engineering / methods*
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Recombinant Proteins / biosynthesis
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Seeds / metabolism
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Triticum / embryology
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Triticum / genetics
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Triticum / metabolism*
Substances
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Arabidopsis Proteins
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Detergents
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Liposomes
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Membrane Transport Proteins
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Recombinant Proteins
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phosphate translocator 1, Arabidopsis