Synthesis of functional proteins within liposomes

Methods Mol Biol. 2010;607:243-56. doi: 10.1007/978-1-60327-331-2_20.

Abstract

In living cells, biochemical reaction systems are enclosed in the small lipidic compartments. To experimentally simulate various biochemical reactions occurring in extant cells, cell-sized lipid vesicles (liposomes) are used to reconstruct an artificial model cell. We present methods for encapsulation of the protein synthesis system inside liposomes and for measurement of the in liposome synthesis reaction using a fluorescence-activated cell sorter. These techniques would enable us to perform detailed analysis of the biochemical reactions occurring in the microcompartments and have the potential to reveal the role of compartmentalization in cellular systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell-Free System
  • Flow Cytometry
  • Green Fluorescent Proteins / biosynthesis*
  • Green Fluorescent Proteins / genetics
  • Humans
  • Liposomes*
  • Protein Biosynthesis*
  • Protein Engineering / methods*
  • Recombinant Proteins / biosynthesis
  • beta-Galactosidase / biosynthesis*
  • beta-Galactosidase / genetics

Substances

  • Liposomes
  • Recombinant Proteins
  • Green Fluorescent Proteins
  • beta-Galactosidase