A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA

Masaki Hagihara; Keisuke Yoneda; Hiroaki Yabuuchi; Yasushi Okuno; Kazuhiko Nakatani

doi:10.1016/j.bmcl.2010.01.158

A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA

Bioorg Med Chem Lett. 2010 Apr 1;20(7):2350-3. doi: 10.1016/j.bmcl.2010.01.158. Epub 2010 Feb 17.

Authors

Masaki Hagihara¹, Keisuke Yoneda, Hiroaki Yabuuchi, Yasushi Okuno, Kazuhiko Nakatani

Affiliation

¹ Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University, Ibaraki 567-0047, Japan.

PMID: 20206514
DOI: 10.1016/j.bmcl.2010.01.158

Abstract

Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

G-Quadruplexes*
Humans
RNA, Messenger / genetics*
RNA-Directed DNA Polymerase / metabolism*
Untranslated Regions*

Substances

RNA, Messenger
Untranslated Regions
RNA-Directed DNA Polymerase