A highly controllable reconstituted cell-free system--a breakthrough in protein synthesis research

Curr Pharm Biotechnol. 2010 Apr;11(3):267-71. doi: 10.2174/138920110791111889.

Abstract

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Publication types

  • Review

MeSH terms

  • Cell-Free System / chemistry*
  • Cell-Free System / metabolism*
  • Protein Engineering / trends*
  • Recombinant Proteins / chemical synthesis*
  • Recombinant Proteins / metabolism*
  • Technology, Pharmaceutical / trends*

Substances

  • Recombinant Proteins