Genetic analysis of strains of Japanese Encephalitis Virus isolated from swine in central China

Virus Genes. 2010 Jun;40(3):357-61. doi: 10.1007/s11262-010-0464-9. Epub 2010 Mar 6.

Abstract

In this study, four strains of Japanese Encephalitis Virus (JEV) were isolated from the cerebrospinal fluid of aborted fetuses or stillborn piglets collected randomly from a number of piggeries in central China. The E genes were cloned by RT-PCR and sequenced. Phylogenetic analysis was performed with 48 JEV isolates previously reported in China and other countries, and showed that all four isolates can be classified into the subcluster of genotype III. The results strongly suggest that the genotype III of JEV is the major variant currently circulating in the swinery of central China.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cerebrospinal Fluid / virology
  • China
  • Cluster Analysis
  • Encephalitis Virus, Japanese / classification
  • Encephalitis Virus, Japanese / genetics*
  • Encephalitis Virus, Japanese / isolation & purification*
  • Encephalitis, Japanese / veterinary*
  • Encephalitis, Japanese / virology
  • Membrane Glycoproteins / genetics
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics
  • Sequence Analysis, DNA
  • Sequence Homology
  • Swine / virology*
  • Swine Diseases / virology*
  • Viral Envelope Proteins / genetics

Substances

  • Membrane Glycoproteins
  • RNA, Viral
  • Viral Envelope Proteins
  • glycoprotein E, Japanese encephalitis virus

Associated data

  • GENBANK/GQ336809
  • GENBANK/GQ845081
  • GENBANK/GQ845082
  • GENBANK/GQ845083