Moderate hypothermia suppressed excessive generation of superoxide anion radical and inflammatory reactions in blood and liver in heatstroke: laboratory study in rats

Free Radic Res. 2010 Apr;44(4):462-72. doi: 10.3109/10715761003610752.


Abstract The study was performed to demonstrate superoxide radical (O(2).-) generation, systemic inflammation and liver injury caused by heatstroke and to reveal suppressive effects of moderate hypothermia. Heatstroke was defined as achieving pharyngeal temperature of 40 degrees C with arterial pressure reduction. Heatstroke rats were divided to four groups by the temperature after the onset; 40 degrees C, 37 degrees C, 32 degrees C and sham-treated with 37 degrees C. O(2).- current was measured continuously in the right atrium using an electrochemical O(2).- sensor. The O(2).- current increased in all groups except for the sham-treated group during the induction. After the onset of heatstroke, the O(2).- current was suppressed with temperature-dependency. Plasma and liver high-mobility group box 1, intercellular adhesion molecule-1, plasma aspartate aminotransferase and alanine aminotransferase were also suppressed with the suppression of O(2).- generation. Therefore, excessive O(2).- generation might be a key factor in heatstroke and the suppression with moderate hypothermia would be a therapeutic modality.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acidosis, Lactic / immunology
  • Acidosis, Lactic / metabolism
  • Acidosis, Lactic / prevention & control
  • Alanine Transaminase / blood
  • Animals
  • Aspartate Aminotransferases / blood
  • Blood Pressure
  • Disease Models, Animal
  • HMGB1 Protein / metabolism
  • Heat Stroke / immunology
  • Heat Stroke / metabolism
  • Heat Stroke / physiopathology
  • Heat Stroke / therapy*
  • Hypothermia, Induced*
  • Inflammation Mediators / blood
  • Inflammation Mediators / metabolism*
  • Intercellular Adhesion Molecule-1 / metabolism
  • Ion-Selective Electrodes
  • Liver / immunology
  • Liver / metabolism*
  • Male
  • Rats
  • Rats, Wistar
  • Superoxides / blood
  • Superoxides / metabolism*
  • Time Factors


  • HMGB1 Protein
  • Hbp1 protein, rat
  • Inflammation Mediators
  • Superoxides
  • Intercellular Adhesion Molecule-1
  • Aspartate Aminotransferases
  • Alanine Transaminase