Glycogen synthesis by amylosucrase from Neisseria perflava

Can J Microbiol. 1977 Sep;23(9):1303-7. doi: 10.1139/m77-196.


Amylosucrase (sucrose:1,4-alpha-D-glucan 4-alpha-glucosyltransferase; EC which mediates the transfer of the glucosyl moiety of sucrose to a growing alpha-1,4-glucan chain is a constitutive enzyme of Neisseria perflava. The products of enzymic action are insoluble glycogenlike polysaccharides and fructose, the latter being a competitive inhibitor of the enzyme (Ki=20 mM). The enzyme is extremely stable and appears to bind very tightly to its polymerized product. Properties of product-bound enzyme reflect those of the native complex.

MeSH terms

  • Cell-Free System
  • Drug Stability
  • Fructose / biosynthesis*
  • Glucosyltransferases / metabolism*
  • Glycogen / biosynthesis*
  • Hydrogen-Ion Concentration
  • Neisseria / enzymology*
  • Neisseria / metabolism
  • Sucrose / metabolism


  • Fructose
  • Sucrose
  • Glycogen
  • Glucosyltransferases