Although previous studies have shown the effectiveness of Koryo hand therapy (KHT) in alleviating menopausal symptoms in middle-aged women, few studies have examined physiological data to evaluate the effect of KHT on menopausal symptoms. The purpose of this study was to examine the effects of KHT on biological markers such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E(2)), and self-reported symptoms. A two-group pre-post test design was used in the study. Data were collected from October 18 to December 25, 2006. The participants were 23 people in the experimental group and 21 people in the control group. The experimental group received KHT three times per week for a total of 8 weeks. Findings show that the levels of FSH and LH were significantly different in both groups, but levels of E(2) were statistically insignificant. The data support the hypothesis that KHT may have a role in reducing menopausal symptoms in the experimental group. KHT is effective in alleviating certain menopausal symptoms and affects the serum level of FSH and LH as shown by physiological evidence and participants' self-reported symptoms. Further studies are necessary to examine the effects of KHT through more objective data.