Pax-3, a novel murine DNA binding protein expressed during early neurogenesis

EMBO J. 1991 May;10(5):1135-47.


We describe the isolation and characterization of Pax-3, a novel murine paired box gene expressed exclusively during embryogenesis. Pax-3 encodes a 479 amino acid protein with an Mr of 56 kd containing both a paired domain and a paired-type homeodomain. The Pax-3 protein is a DNA binding protein that specifically recognizes the e5 sequence present upstream of the Drosophila even-skipped gene. Pax-3 transcripts are first detected in 8.5 day mouse embryos where they are restricted to the dorsal part of the neuroepithelium and to the adjacent segmented dermomyotome. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme of 10 and 11 day embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Drosophila / embryology
  • Drosophila / genetics*
  • Gene Expression
  • Mesoderm / metabolism
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Neural Crest / growth & development
  • Neural Crest / metabolism*
  • Neural Crest / ultrastructure
  • PAX3 Transcription Factor
  • Paired Box Transcription Factors
  • Transcription Factors / genetics
  • Transcription, Genetic


  • DNA-Binding Proteins
  • PAX3 Transcription Factor
  • Paired Box Transcription Factors
  • Transcription Factors
  • Pax3 protein, mouse

Associated data

  • GENBANK/M55398
  • GENBANK/M55399
  • GENBANK/M55400
  • GENBANK/M55401
  • GENBANK/M55402
  • GENBANK/M65224
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/X59082
  • GENBANK/X59358