Subrepeats of rDNA intergenic spacer present as prominent independent satellite DNA in Vigna radiata but not in Vigna angularis

Gene. 1991 Mar 1;99(1):63-8. doi: 10.1016/0378-1119(91)90034-9.

Abstract

Subrepeats located in the rDNA intergenic spacer are also present as independently occurring, tandemly arranged satellite DNA clusters in the genome of Vigna radiata (mung bean). These 174-bp satellite repeats are identified as non-rDNA repeats by the presence of an AluI site. In the closely related Vigna angularis (adzuki bean), 174-bp repeats characterized by an AluI site occur in the rDNA with high sequence homology to the V. radiata rDNA subrepeats. A part of the 174-bp element that shows high similarity to a Xenopus terminator box (T2/T3) is slightly modified in V. angularis. However, a characteristic stem-loop structure can be formed, as in the case of V. radiata. Two highly conserved 12-bp regions occur within the 174-bp rDNA repeats of the two plants investigated. One of these 12-bp stretches exhibits some sequence identity to an element repeated twice in the 325-bp repeats in the intergenic spacer region of Vicia faba (broad bean).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Base Sequence
  • DNA, Ribosomal / genetics*
  • DNA, Ribosomal / isolation & purification
  • DNA, Satellite / genetics*
  • DNA, Satellite / isolation & purification
  • Fabaceae / genetics*
  • Introns*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Plants, Medicinal*
  • Plasmids
  • Repetitive Sequences, Nucleic Acid
  • Species Specificity
  • Transcription, Genetic
  • Xenopus

Substances

  • DNA, Ribosomal
  • DNA, Satellite

Associated data

  • GENBANK/X16184
  • GENBANK/X17209
  • GENBANK/X17210