Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 10, 77

The PTS Transporters of Lactobacillus Gasseri ATCC 33323

Affiliations

The PTS Transporters of Lactobacillus Gasseri ATCC 33323

Alyssa L Francl et al. BMC Microbiol.

Abstract

Background: Lactobacilli can utilize a variety of carbohydrates which reflects the nutrient availability in their respective environments. A common lactobacilli in the human gastrointestinal tract, Lactobacillus gasseri, was selected for further study. The currently available annotation of the L. gasseri ATCC 33323 genome describes numerous putative genes involved in carbohydrate utilization, yet the specific functions of many of these genes remain unknown.

Results: An enzyme I (EI) knockout strain revealed that a functional phosphotransferase transporter system (PTS) is required to ferment at least 15 carbohydrates. Analysis of the L. gasseri ATCC 33323 genome identified fifteen complete (containing all of the necessary subunits) PTS transporters. Transcript expression profiles in response to various carbohydrates (glucose, mannose, fructose, sucrose and cellobiose) were analyzed for the fifteen complete PTS transporters in L. gasseri. PTS 20 was induced 27 fold in the presence of sucrose and PTS 15 was induced 139 fold in the presence of cellobiose. No PTS transporter was induced by glucose, fructose or mannose. Insertional inactivation of PTS 15 and PTS 20 significantly impaired growth on cellobiose and sucrose, respectively. As predicted by bioinformatics, insertional inactivation of PTS 21 confirmed its role in mannose utilization.

Conclusions: The experiments revealed the extensive contribution of PTS transporters to carbohydrate utilization by L. gasseri ATCC 33323 and the general inadequacy of the annotated sugar specificity of lactobacilli PTS transporters.

Figures

Figure 1
Figure 1
Relative fold changes of the complete PTS transporters in L. gasseri ATCC 33323. Cells grown in semi-synthetic MRS + selected carbohydrate were compared to cells grown in semi-synthetic MRS + fructose. Selected carbohydrates were sucrose (A), cellobiose (B), glucose (C) and mannose (D). RNA was extracted from log phase cells and subjected to two-step real-time PCR. Results are the average of three independent experiments, and error bars indicate standard deviations.
Figure 2
Figure 2
Growth curves of selected L. gasseri strains. Growth curves of MJM99 (blue), MJM100 (red), MJM101 (green), MJM75 (purple), NCK334 (black) and an uninoculated control (orange) grown in semi-synthetic MRS + selected carbohydrate. Selected carbohydrates were sucrose (A), cellobiose (B), glucose (C) and mannose (D). Results are the average of duplicate wells from one of three independent experiments.

Similar articles

See all similar articles

Cited by 12 PubMed Central articles

See all "Cited by" articles

References

    1. Kandler O. Carbohydrate metabolism in lactic acid bacteria. Antonie van Leeuwenhoek. 1983;49(3):209. doi: 10.1007/BF00399499. - DOI - PubMed
    1. Hutkins RW. Microbiology and Technology of Fermented Foods. 1. Chicago, Ill.; Ames, Iowa: IFT Press; Blackwell Pub; 2006.
    1. Azcarate-Peril MA, Altermann E, Goh YJ, Tallon R, Sanozky-Dawes RB, Pfeiler EA, O'Flaherty S, Buck BL, Dobson A, Duong T, Miller MJ, Barrangou R, Klaenhammer TR. Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism. Appl Environ Microbiol. 2008;74(15):4610. doi: 10.1128/AEM.00054-08. - DOI - PMC - PubMed
    1. Reuter G. The Lactobacillus and Bifidobacterium microflora of the human intestine: composition and succession. Curr Issues Intest Microbiol. 2001;2(2):43. - PubMed
    1. Liévin-Le Moal V, Servin AL. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota. Clin Microbiol Rev. 2006;19(2):315. doi: 10.1128/CMR.19.2.315-337.2006. - DOI - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

Feedback