Interaction between Ataxin-2 Binding Protein 1 and Cubitus-interruptus during wing development in Drosophila

Dev Biol. 2010 May 15;341(2):389-99. doi: 10.1016/j.ydbio.2010.02.039. Epub 2010 Mar 11.

Abstract

Animal growth and development is dependent on reiterative use of key signaling pathways such as Hedgehog (Hh) pathway. It is widely believed that Cubitus-interruptus (Ci) mediates all functions of Hh pathway. Here we report that CG32062, the Drosophila homologue of Ataxin-2 Binding Protein 1 (dA2BP1), functions as a cofactor of Ci to specify intervein region between L3 and L4 veins of the adult wing. Specifically, Ci-mediated transactivation of knot/collier (kn) in this region of the developing wing imaginal disc is dependent on dA2BP1 function. Protein interaction studies and chromatin-immunoprecipiation experiments suggest that Ci helps dA2BP1 to bind kn promoter, which in turn may help Ci to activate kn expression. These results suggest a mechanism by which Ci may activate targets such as kn, which do not have classical Ci/Gli-binding sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA-Binding Proteins / metabolism*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism
  • RNA-Binding Proteins / metabolism*
  • Transcription Factors / metabolism*
  • Wings, Animal / metabolism

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • RNA-Binding Proteins
  • Rbfox1 protein, Drosophila
  • Transcription Factors
  • ci protein, Drosophila
  • kn protein, Drosophila