The RGD containing site of the mouse laminin A chain is active for cell attachment, spreading, migration and neurite outgrowth

J Cell Physiol. 1991 Mar;146(3):451-9. doi: 10.1002/jcp.1041460316.

Abstract

The laminin A chain has been sequenced by cDNA cloning and was found to contain an RGD sequence. Synthetic peptides containing the RGD sequence and flanking amino acids were active in mediating cell adhesion, spreading, migration, and neurite outgrowth. Furthermore, endothelial cell attachment to a laminin substrate was inhibited by an RGD-containing synthetic peptide. Antisera against the integrin (fibronectin) receptor, and monoclonal antibody to the integrin, VLA-6, inhibited cell interaction with laminin, as well as with peptides containing an RGD sequence. These results suggest that the RGD containing site of laminin is active and interacts with the integrin family of receptors in certain cells.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / physiology
  • Axons / drug effects
  • Axons / physiology*
  • Axons / ultrastructure
  • Cattle
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology*
  • Cell Movement / drug effects
  • Cell Movement / physiology*
  • Cells, Cultured
  • Endothelium / cytology
  • Endothelium / physiology
  • Fibrosarcoma / pathology
  • Fibrosarcoma / physiopathology
  • Humans
  • Hybridomas / pathology
  • Hybridomas / physiology
  • Integrins / immunology
  • Laminin / analysis
  • Laminin / chemistry
  • Laminin / physiology*
  • Melanoma / pathology
  • Melanoma / physiopathology
  • Mice
  • Molecular Sequence Data
  • Oligopeptides / analysis
  • Oligopeptides / chemistry
  • Oligopeptides / physiology*
  • Precipitin Tests
  • Rhabdomyosarcoma / pathology
  • Rhabdomyosarcoma / physiopathology

Substances

  • Amino Acids
  • Antibodies, Monoclonal
  • Integrins
  • Laminin
  • Oligopeptides
  • arginyl-glycyl-aspartic acid