Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34+38- compartment

Leuk Res. 2010 Oct;34(10):1287-95. doi: 10.1016/j.leukres.2010.02.012. Epub 2010 Mar 12.


The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase 1 / analysis*
  • Antigens, CD34 / analysis*
  • Cell Line, Tumor
  • Chromosomes, Human, Pair 4
  • Comparative Genomic Hybridization
  • Gene Expression Profiling
  • Humans
  • Immunophenotyping
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / immunology
  • MicroRNAs / analysis*
  • Proto-Oncogene Proteins c-kit / genetics


  • Antigens, CD34
  • MicroRNAs
  • Proto-Oncogene Proteins c-kit
  • ADP-ribosyl Cyclase 1