Cloning and characterization of a novel gene that encodes (S)-beta-bisabolene synthase from ginger, Zingiber officinale

Planta. 2010 Jun;232(1):121-30. doi: 10.1007/s00425-010-1137-6. Epub 2010 Mar 13.

Abstract

Ginger, Zingiber officinale Roscoe, contains a fragrant oil mainly composed of sesquiterpenes and monoterpenes. We isolated a cDNA that codes for a sesquiterpene synthase from young rhizomes of ginger, Z. officinale Roscoe, Japanese cultivar "Kintoki". The cDNA, designated ZoTps1, potentially encoded a protein that comprised 550 amino acid residues and exhibited 49-53% identity with those of the sesquiterpene synthases already isolated from the genus Zingiber. Recombinant Escherichia coli cells, in which ZoTps1 was coexpressed along with genes for D-mevalonate utilization, resulted in the production of a sesquiterpene (S)-beta-bisabolene exclusively with a D-mevalonolactone supplement. This result indicated that ZoTps1 was the (S)-beta-bisabolene synthase gene in ginger. ZoTPS1 was suggested to catalyze (S)-beta-bisabolene formation with the conversion of farnesyl diphosphate to nerolidyl diphosphate followed by the cyclization between position 1 and 6 carbons. The ZoTps1 transcript was detected in young rhizomes, but not in leaves, roots and mature rhizomes of the ginger "Kintoki".

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkyl and Aryl Transferases / chemistry
  • Alkyl and Aryl Transferases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • Gas Chromatography-Mass Spectrometry
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Ginger / genetics*
  • Molecular Sequence Data
  • Monocyclic Sesquiterpenes
  • Phylogeny
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Sesquiterpenes / metabolism*

Substances

  • DNA Primers
  • Monocyclic Sesquiterpenes
  • Sesquiterpenes
  • Alkyl and Aryl Transferases
  • beta-bisabolene

Associated data

  • GENBANK/AB511914