Radionuclide angiography with technetium-99m in vivo labeled erythrocytes does not lead to induction of mutations in the HPRT gene of human T-lymphocytes

F J van Dam; J A Camps; V M Woldring; A T Natarajan; E E van der Wall; A H Zwinderman; P H Lohman; E K Pauwels; A D Tates

Radionuclide angiography with technetium-99m in vivo labeled erythrocytes does not lead to induction of mutations in the HPRT gene of human T-lymphocytes

J Nucl Med. 1991 May;32(5):814-8.

Authors

F J van Dam¹, J A Camps, V M Woldring, A T Natarajan, E E van der Wall, A H Zwinderman, P H Lohman, E K Pauwels, A D Tates

Affiliation

¹ MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.

PMID: 2022988

Abstract

Mutant frequencies were measured in T-lymphocytes of patients undergoing radionuclide angiography with erythrocytes labeled in vivo with technetium-99m. Blood from 13 patients was sampled before and after (8-120 days) an injection with 750 MBq technetium-99m. Frequencies of HPRT- mutants were measured with the T-cell cloning method. Results indicated that the mean frequency of mutants after treatment was significantly below that measured before exposure. Thus, in contrast to published data, our results do not support the conclusion that radionuclide angiography with technetium-99m induces HPRT- mutations. Further analysis of our data indicated that the decrease in mutant frequency after exposure can be accounted for by an effect of cloning efficiency.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Erythrocytes
Female
Gated Blood-Pool Imaging*
Humans
Hypoxanthine Phosphoribosyltransferase / genetics*
Male
Middle Aged
Mutation*
Radiation Genetics
T-Lymphocytes / radiation effects*
Technetium*

Substances

Technetium
Hypoxanthine Phosphoribosyltransferase