Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters

J Am Chem Soc. 2010 Aug 11;132(31):10628-9. doi: 10.1021/ja101387b.


Lipoproteins are a largely uncharacterized class of proteins in bacteria. In this study, metabolic labeling of bacteria with fatty acid chemical reporters allowed rapid profiling of lipid-modified proteins. We identified many candidate lipoproteins in Escherichia coli and detected a novel modification on YjgF. This chemical approach should facilitate future characterization of lipoproteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry*
  • Escherichia coli / chemistry
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / metabolism
  • Fatty Acids / chemistry*
  • Lipoproteins / chemistry*
  • Lipoproteins / metabolism
  • Molecular Structure


  • Alkynes
  • Escherichia coli Proteins
  • Fatty Acids
  • Lipoproteins