A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet

Anal Biochem. 2010 Jul 1;402(1):99-101. doi: 10.1016/j.ab.2010.03.017. Epub 2010 Mar 15.

Abstract

We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / analysis*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Rosaniline Dyes*
  • Sensitivity and Specificity
  • Staining and Labeling / economics
  • Staining and Labeling / methods*
  • Viral Proteins / genetics

Substances

  • E protein, bacteriophage X174
  • Rosaniline Dyes
  • Viral Proteins
  • DNA
  • ethyl violet