BIOMED-2 protocols to detect clonal immunoglobulin and T-cell receptor gene rearrangements in B- and T-cell lymphomas in southern Taiwan

Leuk Lymphoma. 2010 Apr;51(4):650-5. doi: 10.3109/10428191003660631.


Monoclonal expansions of immunoglobulin (Ig) and T-cell receptor (TCR) genes are, respectively, important markers for B- and T-cell malignancies. We used BIOMED-2 protocols to detect clonality of these genes in B- (BCL) and T-cell lymphoma (TCL) in southern Taiwan. Heteroduplex analysis was used after PCR reactions. Ig/TCR clonality rates were 95% (22 in 23 cases) for BCL and 76% (19 in 25 cases) for TCL. These results reveal overall satisfactory detection rates for both BCL and TCL. None of the four natural killer (NK)/T-cell lymphomas detected showed TCR gene clonality, which suggested that BIOMED-2 protocols distinguished the NK/T-cell lymphoma from TCL. In addition, three of the five tested precursor T-cell lymphoblastic lymphomas showed no TCR gene clonality, probably because the immature T-cells in this type of TCL had not undergone TCR gene rearrangements. We conclude that BIOMED-2 protocols are suitable for detecting Ig and TCR clonalities in B- and T-cell malignancies in southern Taiwan.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Clinical Protocols*
  • Clone Cells / immunology*
  • Clone Cells / metabolism
  • DNA Mutational Analysis / methods
  • Gene Rearrangement, B-Lymphocyte* / genetics
  • Gene Rearrangement, T-Lymphocyte* / genetics
  • Genes, T-Cell Receptor / genetics
  • Humans
  • Immunoglobulins / analysis
  • Immunoglobulins / genetics
  • Lymphoma, B-Cell / diagnosis
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / immunology
  • Lymphoma, T-Cell / diagnosis
  • Lymphoma, T-Cell / genetics*
  • Lymphoma, T-Cell / immunology
  • Polymerase Chain Reaction / methods
  • Taiwan


  • Immunoglobulins