Histological changes in the pancreas following administration of ethanolic extract of Alchornea cordifolia leaf in alloxan-induced diabetic Wistar rats

Niger J Physiol Sci. 2009 Dec;24(2):153-5. doi: 10.4314/njps.v24i2.52927.


Twenty-four wistar rats weighing between 150 and 170 g were used in this research work. They were divided into four groups A, B, C, and D of six rats each. Diabetes was induced in Groups B, C, and D using a single intraperitoneal injection of 150 mg/kg of Alloxan after an overnight fast. Group A served as the normal control while Group B served as the diabetic control and each received 0.5 ml of normal saline daily. Groups C and D received 250 mg/kg/day and 500 mg/kg /day of the plant extract respectively through orogastric intubation. All the animals were given normal rat chow and water freely. The experiment lasted for 28 days. The animals were anaesthetized using chloroform inhalation and the peritoneum stripped open and the pancreas removed and prepared for histological observation using haematoxylin and eosin staining technique. Histology showed regenerative changes of pancreatic islet cell at a dose of 500 mg/kg/day.

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / pathology
  • Ethanol / chemistry
  • Euphorbiaceae*
  • Hypoglycemic Agents / pharmacology*
  • Insulin-Secreting Cells / drug effects
  • Insulin-Secreting Cells / pathology
  • Pancreas / drug effects*
  • Pancreas / pathology
  • Plant Extracts / pharmacology*
  • Plant Leaves
  • Rats
  • Rats, Wistar
  • Regeneration / drug effects
  • Solvents / chemistry
  • Time Factors


  • Hypoglycemic Agents
  • Plant Extracts
  • Solvents
  • Ethanol