Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

P L Urban; A Amantonico; S R Fagerer; P Gehrig; R Zenobi

doi:10.1039/b925433a

Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

Chem Commun (Camb). 2010 Apr 7;46(13):2212-4. doi: 10.1039/b925433a. Epub 2010 Feb 25.

Authors

P L Urban¹, A Amantonico, S R Fagerer, P Gehrig, R Zenobi

Affiliation

¹ Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.

PMID: 20234909
DOI: 10.1039/b925433a

Abstract

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyl Coenzyme A / chemistry
Adenosine Diphosphate / chemistry
Adenosine Triphosphate / chemistry
Coenzyme A / chemistry
Enzymes / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Enzymes
Adenosine Diphosphate
Acetyl Coenzyme A
Adenosine Triphosphate
Coenzyme A