Antioxidant and NF-κB inhibitory constituents isolated from Morchella esculenta

Nat Prod Res. 2011 Sep;25(15):1412-7. doi: 10.1080/14786410802425746. Epub 2011 Jul 8.


Morchella esculenta Pers. (Morchellaceae) was investigated for its antioxidant activity by measuring the intracellular reactive oxygen species in HT-29 colon cancer cells. The methylene chloride extract, which showed the highest antioxidant activity, led to the isolation of four fungal sterols, 1-4, and trilinolein (5), in addition to methyl myristate (6), 1-linoleoylglycerol (7), and ceramide (8). The isolated compounds were identified through the analysis of various spectroscopic methods. In the hydroxyl radical assay, 5-dihydroergosterol exhibited significant antioxidant activity. All compounds isolated were also tested using an enzyme-based Elisa NF-κB assay. Fungal sterols and trilinolein showed significant inhibition of NF-κB activation in the NF-κB assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Ascomycota / chemistry*
  • Chromatography, Thin Layer
  • Enzyme-Linked Immunosorbent Assay
  • HT29 Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / metabolism
  • Reactive Oxygen Species / metabolism
  • Spectrophotometry, Ultraviolet


  • Antioxidants
  • NF-kappa B
  • Reactive Oxygen Species