Cytosolic protein interactions of the schizophrenia susceptibility gene dysbindin

J Neurochem. 2010 Jun;113(6):1491-503. doi: 10.1111/j.1471-4159.2010.06690.x. Epub 2010 Mar 14.


Using immunoprecipitation, mass spectrometry, and western blot analysis we investigated cytosolic protein interactions of the schizophrenia susceptibility gene dysbindin in mammalian cells. We identified novel interactions with members of the exocyst, dynactin and chaperonin containing T-complex protein complexes, and we confirmed interactions reported previously with all members of the biogenesis of lysosome-related organelles complex-1 and the adaptor-related protein complex 3. We report interactions between dysbindin and the exocyst and dynactin complex that confirm a link between two important schizophrenia susceptibility genes: dysbindin and disrupted-in-schizophrenia-1. To expand upon this network of interacting proteins we also investigated protein interactions for members of the exocyst and dynactin complexes in mammalian cells. Our results are consistent with the notion that impairment of aspects of the synaptic vesicle life cycle may be a pathogenic mechanism in schizophrenia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line, Transformed
  • Chi-Square Distribution
  • Computational Biology
  • Dysbindin
  • Dystrophin-Associated Proteins
  • Exocytosis / genetics
  • Green Fluorescent Proteins / genetics
  • Humans
  • Immunoprecipitation / methods
  • Mass Spectrometry / methods
  • Mutation
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Protein Binding / genetics
  • Protein Transport / genetics
  • Schizophrenia / genetics
  • Synaptic Vesicles / genetics
  • Synaptic Vesicles / metabolism*
  • Transfection / methods


  • Carrier Proteins
  • DISC1 protein, human
  • DTNBP1 protein, human
  • Dysbindin
  • Dystrophin-Associated Proteins
  • Nerve Tissue Proteins
  • Green Fluorescent Proteins