Characterization of SVEP1, KIAA, and SRPX2 in an in vitro cell culture model of endotoxemia

Cell Immunol. 2010;263(1):65-70. doi: 10.1016/j.cellimm.2010.02.017. Epub 2010 Feb 26.

Abstract

To assess the influence of unknown factors in endotoxemia, a conditioned medium, achieved by the stimulation of THP1 monocytes with lipopolysaccharide (LPS) [4h], was used for the stimulation of human umbilical vein endothelial cells (HUVECs) [16h]. SVEP1, KIAA0247, and SRPX2 were selected after microarray analysis. To study their possible functions, siRNAs of SVEP1, KIAA0247, or SRPX2 were used for the transfection of HUVECs and cells were stimulated with conditioned medium [16h]. Inhibition of SVEP1 expression resulted in an increase of soluble intercellular adhesion molecule (sICAM) 1 (10%) and soluble E-selectin (sE-selectin) (19%). Inhibition of SRPX2 led to an increase of sICAM (11%) and sE-selectin (14%). KIAA0247 negative HUVECs showed a decrease in monocyte chemoattractant protein (MCP) 1 of 16%. SVEP1 and SRPX2 seemed to act as regulators of ICAM1 and E-selectin shedding and influence the expression of membrane bound adhesion molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism*
  • Cell Line
  • Complement System Proteins / genetics
  • Complement System Proteins / immunology
  • Complement System Proteins / metabolism*
  • Culture Media, Conditioned
  • Cytokines / metabolism
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects
  • Endothelial Cells / immunology
  • Endothelial Cells / metabolism*
  • Endotoxemia / genetics
  • Endotoxemia / immunology
  • Endotoxemia / metabolism*
  • Endotoxemia / pathology
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / immunology
  • Humans
  • Inflammation
  • L-Selectin / immunology
  • L-Selectin / metabolism
  • Lipopolysaccharides / immunology
  • Lipopolysaccharides / metabolism
  • Membrane Proteins
  • Microarray Analysis
  • Monocytes / immunology
  • Monocytes / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / immunology
  • Nerve Tissue Proteins / metabolism*
  • RNA, Small Interfering / genetics

Substances

  • Cell Adhesion Molecules
  • Culture Media, Conditioned
  • Cytokines
  • Lipopolysaccharides
  • Membrane Proteins
  • Nerve Tissue Proteins
  • RNA, Small Interfering
  • SRPX2 protein, human
  • SUSD6 protein, human
  • SVEP1 protein, human
  • L-Selectin
  • Complement System Proteins