The evolutionarily conserved Krüppel-associated box domain defines a subfamily of eukaryotic multifingered proteins

Proc Natl Acad Sci U S A. 1991 May 1;88(9):3608-12. doi: 10.1073/pnas.88.9.3608.


We have previously shown that the human genome includes hundreds of genes coding for putative factors related to the Krüppel zinc-finger protein, which regulates Drosophila segmentation. We report herein that about one-third of these genes code for proteins that share a very conserved region of about 75 amino acids in their N-terminal nonfinger portion. Homologous regions are found in a number of previously described finger proteins, including mouse Zfp-1 and Xenopus Xfin. We named this region the Krüppel-associated box (KRAB). This domain has the potential to form two amphipathic alpha-helices. Southern blot analysis of "zoo" blots suggests that the Krüppel-associated box is highly conserved during evolution. Northern blot analysis shows that these genes are expressed in most adult tissues and are down-regulated during in vitro terminal differentiation of human myeloid cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • Gene Library
  • Humans
  • Juvenile Hormones / chemistry*
  • Juvenile Hormones / genetics
  • Kruppel-Like Transcription Factors
  • Molecular Sequence Data
  • Repressor Proteins*
  • Transcription Factors*
  • Tumor Cells, Cultured
  • Zinc Fingers*


  • DNA-Binding Proteins
  • Juvenile Hormones
  • Kruppel-Like Transcription Factors
  • Repressor Proteins
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/M61866
  • GENBANK/M61867
  • GENBANK/M61868
  • GENBANK/M61869
  • GENBANK/M61870
  • GENBANK/M61871
  • GENBANK/M61872
  • GENBANK/M61873