The binding site on SV40 DNA for a T antigen-related protein

Cell. 1978 Jan;13(1):165-79. doi: 10.1016/0092-8674(78)90147-2.


A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae
  • Antigens, Neoplasm*
  • Antigens, Viral*
  • Base Sequence
  • Binding Sites
  • DNA Replication
  • DNA, Viral / metabolism*
  • Defective Viruses
  • Deoxyribonucleases / metabolism
  • Simian virus 40* / immunology
  • Viral Proteins / isolation & purification
  • Viral Proteins / metabolism*


  • Antigens, Neoplasm
  • Antigens, Viral
  • DNA, Viral
  • Viral Proteins
  • Deoxyribonucleases

Associated data

  • GENBANK/J02400
  • GENBANK/J02402
  • GENBANK/J02403
  • GENBANK/J02406
  • GENBANK/J02407
  • GENBANK/J02408
  • GENBANK/J02409
  • GENBANK/J02410
  • GENBANK/V01380