Molecular analysis of a baculovirus regulatory gene

Virology. 1991 May;182(1):279-86. doi: 10.1016/0042-6822(91)90671-w.

Abstract

To better understand the structure and function of a baculovirus regulatory gene, the nucleotide sequence of IE-N expressed by Autographa californica nuclear polyhedrosis virus was determined. The 2.0-kb PstI-EcoRV restriction fragment (97.5 to 98.9 mu) encodes the upstream regulatory sequences, open reading frame, and downstream sequences of the immediate early IE-N gene. Using a convenient restriction site, the 285-bp promoter of IE-N was divided into two functional regions as defined by transient expression assays of mutant sequences. The sequences of IE-N from -1 to -45 nt encoded a minimal promoter capable of directing low levels of transcription. The minimal promoter was fully responsive to positive regulation by IE-N. The upstream region from -46 to -285 nt contains two direct repeats which increased levels of IE-N gene expression. Computer-assisted translation of the IE-N sequence indicates that this fragment of DNA encodes a single long open reading frame with a predicted molecular weight of 47,000. The amino acid sequence of the predicted protein exhibits three motifs common to transcriptional regulators: a serine-threonine rich region, a proline-rich region, and a polyglutamine tract. IE-N autoregulates its own expression and stimulates both IE-1 and IE-0 in transient assays. The stimulation of IE-1 may account for the augmenting activity of IE-N in the IE-1-mediated trans-activation of the 39K promoter.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Baculoviridae / genetics*
  • Base Sequence
  • DNA Mutational Analysis
  • DNA, Viral / genetics
  • DNA-Binding Proteins*
  • Gene Expression Regulation, Viral
  • Genes, Regulator*
  • Genes, Viral*
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Promoter Regions, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Trans-Activators / genetics*
  • Viral Proteins / genetics
  • Viral Structural Proteins / genetics*

Substances

  • DNA, Viral
  • DNA-Binding Proteins
  • Oligonucleotides
  • Trans-Activators
  • Viral Proteins
  • Viral Structural Proteins
  • Ac-IE-2 protein, Autographa californica nucleopolyhedrovirus

Associated data

  • GENBANK/M59049
  • GENBANK/M59050
  • GENBANK/M59422
  • GENBANK/M64464
  • GENBANK/M64467
  • GENBANK/M64469
  • GENBANK/M64470
  • GENBANK/M65833
  • GENBANK/M65865
  • GENBANK/M76764