ATP-dependent transport for glucuronides in canalicular plasma membrane vesicles

Biochem Biophys Res Commun. 1991 Apr 30;176(2):622-6. doi: 10.1016/s0006-291x(05)80229-3.

Abstract

Using rat liver canalicular plasma membrane vesicles, it has been verified that the transport of p-nitrophenyl glucuronide (NPG) across membranes is an ATP-dependent process; the apparent Km for NPG was 20 microM. S-(2,4-dinitrophenyl)-glutathione (DNP-SG) inhibited NPG uptake dose-dependently, and NPG or testosterone glucuronide did ATP-dependent DNP-SG uptake similarly. These results suggest that transport of glucuronide is mediated by an ATP-dependent glutathione S-conjugate carrier.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Biological Transport
  • Cell Membrane / metabolism
  • Glucuronates / metabolism*
  • Glutathione / analogs & derivatives*
  • Glutathione / metabolism
  • Liver / metabolism*
  • Male
  • Models, Molecular
  • Rats
  • Rats, Inbred Strains
  • Testosterone / analogs & derivatives*
  • Testosterone / metabolism

Substances

  • Glucuronates
  • 4-nitrophenylglucuronide
  • testosterone glucuronate
  • S-(2,4-dinitrophenyl)glutathione
  • Testosterone
  • Adenosine Triphosphate
  • Glutathione