Abstract
A full-length cDNA encoding the human H-protein of the glycine cleavage system has been isolated from a lambda gt11 human fetal liver cDNA library. The cDNA insert was 1091 base pairs with an open reading frame of 519 base pairs which encoded a 125-amino acid mature human H-protein with a 48-amino acid presequence. Human H-protein is 97%, 86%, and 46% identical to the bovine, chicken, and pea H-protein, respectively.
MeSH terms
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Amino Acid Oxidoreductases*
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Amino Acid Sequence
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Animals
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Base Composition
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Base Sequence
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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Cattle
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Chickens
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Cloning, Molecular
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DNA / chemistry*
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Fabaceae / genetics
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Genomic Library
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Glycine / metabolism
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Glycine Decarboxylase Complex H-Protein
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Glycine Dehydrogenase (Decarboxylating)
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Humans
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Hydrolysis
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Liver / chemistry*
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Molecular Sequence Data
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Plants, Medicinal
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Restriction Mapping
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Sequence Homology, Nucleic Acid
Substances
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Carrier Proteins
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Glycine Decarboxylase Complex H-Protein
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DNA
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Amino Acid Oxidoreductases
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Glycine Dehydrogenase (Decarboxylating)
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Glycine
Associated data
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GENBANK/D90312
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GENBANK/D90313
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GENBANK/M62480
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GENBANK/M62481
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GENBANK/M62482
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GENBANK/M69175
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GENBANK/M69176
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GENBANK/M69194
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GENBANK/M72238
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GENBANK/X53944