Cell surface glycosylation changes accompanying immortalization and transformation of normal human mammary epithelial cells

Cancer Lett. 1991 Apr;57(1):27-36. doi: 10.1016/0304-3835(91)90059-q.


Fluorescent lectin binding to cell surfaces was quantitatively analysed by flow cytometry on mortal human breast epithelial cells MCF-10M, the immortalized cell line MCF-10A derived from MCF-10M and sublines of MCF-10A transfected with the neomycin resistance gene (MCF-10Aneo), the c-Ha-ras protooncogene (MCF-10AneoN), or transfected and transformed with the c-Ha-ras activated oncogene (MCF-10AneoT). Immortal MCF-10A cells bound 10-fold more peanut agglutinin (PNA) and soy bean agglutinin (SBA) than did MCF-10M cells. Transformed MCF-10AneoT cells bound approximately ten times more PNA than did non-transformed cells transfected with protooncogene (MCF-10AneoN). Treatment of the transfectants with neuraminidase abrogated the differences in PNA-binding and reduced the differences in SBA binding. SDS-PAGE separation of PNA binding glycoproteins revealed different patterns for all MCF-10A derived sublines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Western
  • Breast
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism*
  • Cell Transformation, Neoplastic*
  • Drug Resistance, Microbial / genetics
  • Epithelium / metabolism
  • Female
  • Flow Cytometry / methods
  • Genes, ras*
  • Glycosylation
  • Humans
  • Lectins
  • Membrane Glycoproteins / isolation & purification
  • Membrane Glycoproteins / metabolism*
  • Neuraminidase / pharmacology
  • Transfection


  • Lectins
  • Membrane Glycoproteins
  • Neuraminidase