NMR studies of a DNA containing 8-hydroxydeoxyguanosine

Nucleic Acids Res. 1991 Apr 11;19(7):1407-12. doi: 10.1093/nar/19.7.1407.

Abstract

The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but the overall structure is much the same as that of the unmodified d(CGCGAATTCGCG) duplex. The structural changes caused by 8-hydroxylation of the deoxyguanosine, if any, are localized near the modification site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Base Composition
  • Base Sequence
  • DNA / chemistry*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / chemistry
  • Hydroxylation
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Temperature

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Deoxyguanosine