Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 252 (5007), 809-17

Zinc finger-DNA Recognition: Crystal Structure of a Zif268-DNA Complex at 2.1 A

Affiliations

Zinc finger-DNA Recognition: Crystal Structure of a Zif268-DNA Complex at 2.1 A

N P Pavletich et al. Science.

Abstract

The zinc finger DNA-binding motif occurs in many proteins that regulate eukaryotic gene expression. The crystal structure of a complex containing the three zinc fingers from Zif268 (a mouse immediate early protein) and a consensus DNA-binding site has been determined at 2.1 angstroms resolution and refined to a crystallographic R factor of 18.2 percent. In this complex, the zinc fingers bind in the major groove of B-DNA and wrap part way around the double helix. Each finger has a similar relation to the DNA and makes its primary contacts in a three-base pair subsite. Residues from the amino-terminal portion of an alpha helix contact the bases, and most of the contracts are made with the guanine-rich strand of the DNA. This structure provides a framework for understanding how zinc fingers recognize DNA and suggests that this motif may provide a useful basis for the design of novel DNA-binding proteins.

Similar articles

See all similar articles

Cited by 547 articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback