Gene conversion in Drosophila and the effects of the meiotic mutants mei-9 and mei-218

Genetics. 1991 Apr;127(4):739-46.

Abstract

Simple meiotic gene conversion tracts produced in wild-type females were compared with those from two meiotic mutants, mei-9 and mei-218. The positions and lengths of conversion tracts were determined by denaturing gradient gels and DNA sequencing. Conversion tracts in wild type averaged 885 base pairs in length, were continuous, and displayed no obvious hot spots of initiation. Some unusual conversion events were found in the mei-218 and mei-9 samples, although most events were indistinguishable from wild-type tracts in their length and continuity.

MeSH terms

  • Animals
  • Blotting, Southern
  • Chromosome Mapping
  • Crossing Over, Genetic
  • Drosophila / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Conversion*
  • Genetic Markers
  • Meiosis / genetics*
  • Mosaicism
  • Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Recombination, Genetic
  • Restriction Mapping

Substances

  • Genetic Markers