Effects of the whole-body cryotherapy on a total antioxidative status and activities of some antioxidative enzymes in blood of patients with multiple sclerosis-preliminary study

J Med Invest. 2010 Feb;57(1-2):168-73. doi: 10.2152/jmi.57.168.


Objective: There is evidence that multiple sclerosis (MS) is not only characterized by immune mediated inflammatory reactions but also by neurodegenerative processes. Neutralization of oxidative stress and excitotoxicity, might represent a therapeutic approach to provide neuroprotection in MS. The purpose of this study was to compare changes in total antioxidative status and activities of chosen antioxidative enzymes, such as: SOD, CAT in erythrocytes of patients with MS before and after using WBCT with control group.

Materials and methods: 32 patients with multiple sclerosis (ICD10-G35) and 20 healthy subjects were recruited for the study. The examined MS group (n=16) was treated with a series of 10 daily exposures in a cryogenic chamber (2-3 min, from -120 degrees C to -110 degrees C) and program of exercises. The control MS group (n=16) had only exercises. Plasma TAS as well as SOD and CAT activities in erythrocytes were measured.

Results: The level of TAS in MS patients was distinctly reduced compared to healthy subjects. After two weeks of WBCT treatment an increase of TAS in the whole examined group (p>0.01) were observed in relation to control MS group. There was not increase of CuZnSOD and CAT activities.

Conclusion: Our results suggest positive antioxidant effects of WBCT as a short-term adjuvant treatment for patients suffered due to MS.

MeSH terms

  • Antioxidants / metabolism*
  • Catalase / blood
  • Cryotherapy*
  • Humans
  • Multiple Sclerosis / metabolism
  • Multiple Sclerosis / therapy*
  • Superoxide Dismutase / blood


  • Antioxidants
  • Catalase
  • Superoxide Dismutase