In vivo study of ethyl-2-cyanoacrylate applied in direct contact with nerves regenerating in a novel nerve-guide

J Mater Sci Mater Med. 2010 Jun;21(6):1979-87. doi: 10.1007/s10856-010-4036-2. Epub 2010 Mar 19.


Stitch suture is still the most recommended method to hold a nerve-guide in place but stitch suture is a well known cause of local inflammatory response. Glues of several kinds have been proposed as an alternative but they are not easy to apply in a real surgical setting. In 2006 authors developed a new concept of nerve-guide termed "NeuroBox" which is double-halved, not-degradable and rigid, and allows the use of cyanoacrylic glues. In this study, Authors analyzed histologically the nerve-glue interface. Wistar rats were used as animal model. In group 1, animals were implanted a NeuroBox to promote the regeneration of an experimentally produced 4 mm gap in the sciatic nerve. In group 2, the gap was left without repair ("sham-operated" group). Group 3 was assembled by harvesting 10 contralateral intact nerves to document the normal anatomy. Semi-thin sections for visible light microscopy and ultra-thin sections for Transmission Electron Microscopy were analyzed. Results showed that application of ethyl-2-cyanoacrylate directly to the epineurium produced no significative insult to the underlining nerve fibers nor impaired nerve regeneration. No regeneration occurred in the "sham-operated" group.

MeSH terms

  • Animals
  • Cyanoacrylates
  • Male
  • Nerve Fibers / physiology
  • Nerve Regeneration / physiology*
  • Peripheral Nerves / surgery
  • Prostheses and Implants
  • Rats
  • Rats, Wistar
  • Regeneration
  • Sciatic Nerve / physiology*
  • Sciatic Nerve / surgery*
  • Sutures


  • Cyanoacrylates
  • ethyl 2-cyanoacrylate