Fate Mapping Reveals Separate Origins of T Cells and Myeloid Lineages in the Thymus

Immunity. 2010 Mar 26;32(3):426-36. doi: 10.1016/j.immuni.2010.03.005. Epub 2010 Mar 18.

Abstract

The cellular differentiation pathway originating from the bone marrow leading to early T lymphocytes remains poorly understood. The view that T cells branch off from a lymphoid-restricted pathway has recently been challenged by a model proposing a common progenitor for T cell and myeloid lineages. We generated interleukin-7 receptor alpha (Il7r) Cre recombinase knockin mice and traced lymphocyte development by visualizing the history of Il7r expression. Il7r fate mapping labeled all T cells but few myeloid cells. More than 85% of T cell progenitors were Il7r reporter(+) and, hence, had arisen from an Il7r-expressing pathway. In contrast, the overwhelming majority of myeloid cells in the thymus were derived from Il7r reporter(-) cells. Thus, lymphoid-restricted progenitors are the major route to T cells, and distinct origins of lymphoid and myeloid lineages represent a fundamental hallmark of hematopoiesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Cell Differentiation
  • Cell Lineage*
  • Mice
  • Myeloid Cells / cytology*
  • Myeloid Cells / immunology*
  • Receptors, Interleukin-7 / genetics
  • Receptors, Interleukin-7 / immunology
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology*
  • Thymus Gland / cytology*
  • Thymus Gland / immunology*

Substances

  • Receptors, Interleukin-7